| Active Players |
| 78 | Bartch, Ben | OG | 07/22/1998 | 27 | 6' 06" | 315 | St. John's (MN) | JAX | 20 04 116 | 6 |
| 51 | Beal Jr., Robert | DE | 08/18/1999 | 26 | 6' 04" | 250 | Georgia | SF | 23 05 173 | 3 |
| 48 | Bethune, Tatum | LB | 02/19/2001 | 24 | 5' 11" | 230 | Florida State | SF | 24 07 251 | 2 |
| 97 | Bosa, Nick | DE | 10/23/1997 | 27 | 6' 04" | 266 | Ohio State | SF | 19 01 002 | 7 |
| 64 | Brendel, Jake | OC | 09/10/1992 | 32 | 6' 04" | 299 | UCLA | DAL FA | 16 CFA | 8 |
| 27 | Brown, Ji'Ayir | S | 01/25/2000 | 25 | 5' 11" | 202 | Penn State | SF | 23 03 087 | 3 |
| 74 | Burford, Spencer | OG/OT | 07/19/2000 | 25 | 6' 04" | 300 | UTSA | SF | 22 04 134 | 4 |
| 75 | Colby, Connor | OG | 01/16/2003 | 22 | 6' 06" | 309 | Iowa | SF | 25 07 249 | R |
| 95 | Collins, Alfred | DT | 10/08/2001 | 23 | 6' 06" | 332 | Texas | SF | 25 02 043 | R |
| 93 | Davis, Kalia | DT | 10/02/1998 | 26 | 6' 01" | 310 | Central Florida | SF | 22 06 220 | 4 |
| 92 | Elliott, Jordan | DT | 11/23/1997 | 27 | 6' 04" | 303 | Missouri | CLE | 20 03 088 | 6 |
| 89 | Farrell, Luke | TE | 10/14/1997 | 27 | 6' 05" | 250 | Ohio State | JAX | 21 05 145 | 5 |
| 84 | Gage Jr., Russell | WR | 01/22/1996 | 29 | 6' 00" | 184 | LSU | ATL | 18 06 194 | 7 |
| 57 | Gifford, Luke | LB | 08/28/1995 | 30 | 6' 03" | 243 | Nebraska | DAL FA | 19 CFA | 7 |
| 0 | Green, Renardo | CB | 11/09/2000 | 24 | 6' 00" | 186 | Florida State | SF | 24 02 064 | 2 |
| 94 | Gross-Matos, Yetur | DE | 02/26/1998 | 27 | 6' 05" | 265 | Penn State | CAR | 20 02 038 | 6 |
| 31 | Guerendo, Isaac | RB | 06/28/2000 | 25 | 6' 00" | 221 | Louisville | SF | 24 04 129 | 2 |
| 61 | Hennessy, Matt | OC/OG | 11/17/1997 | 27 | 6' 04" | 315 | Temple | ATL | 20 03 078 | 5 |
| 47 | Huff, Bryce | DE | 04/17/1998 | 27 | 6' 03" | 255 | Memphis | NYJ FA | 20 CFA | 6 |
| 29 | James, Jordan | RB | 03/29/2004 | 21 | 5' 09" | 208 | Oregon | SF | 25 05 147 | R |
| 96 | Jefferson, Jordan | DT | 09/24/2001 | 23 | 6' 03" | 316 | LSU | JAX | 24 04 116 | 2 |
| 15 | Jennings, Jauan | WR | 07/10/1997 | 28 | 6' 03" | 212 | Tennessee | SF | 20 07 217 | 5 |
| 10 | Jones, Mac | QB | 09/05/1998 | 27 | 6' 03" | 220 | Alabama | NE | 21 01 015 | 5 |
| 44 | Juszczyk, Kyle | FB | 04/23/1991 | 34 | 6' 01" | 235 | Harvard | BAL | 13 04 130 | 13 |
| 85 | Kittle, George | TE | 10/09/1993 | 31 | 6' 04" | 250 | Iowa | SF | 17 05 146 | 9 |
| 2 | Lenoir, Deommodore | CB | 10/06/1999 | 25 | 5' 10" | 200 | Oregon | SF | 21 05 172 | 5 |
| 26 | Lucas, Chase | CB | 03/04/1997 | 28 | 5' 11" | 184 | Arizona State | DET | 22 07 237 | 3 |
| 28 | Luter Jr., Darrell | CB | 04/24/2000 | 25 | 6' 00" | 190 | South Alabama | SF | 23 05 155 | 3 |
| 45 | Martin, Nick | LB | 10/13/2002 | 22 | 5' 11" | 221 | Oklahoma State | SF | 25 03 075 | R |
| 23 | McCaffrey, Christian | RB | 06/07/1996 | 29 | 5' 11" | 210 | Stanford | CAR | 17 01 008 | 9 |
| 68 | McKivitz, Colton | OT | 08/09/1996 | 29 | 6' 06" | 301 | West Virginia | SF | 20 05 153 | 6 |
| 4 | Moody, Jake | PK | 11/23/1999 | 25 | 6' 01" | 210 | Michigan | SF | 23 03 099 | 3 |
| 6 | Moore, Skyy | WR | 09/10/2000 | 24 | 5' 10" | 195 | Western Michigan | KC | 22 02 054 | 4 |
| 7 | Morstead, Thomas | PT | 03/07/1986 | 39 | 6' 04" | 225 | SMU | NO | 09 05 164 | 17 |
| 55 | Moss, Drew | OG/OC | 05/21/2003 | 22 | 6' 03" | 303 | Colorado State | SF FA | 25 CFA | R |
| 33 | Neal, Siran | CB | 08/04/1994 | 31 | 6' 00" | 206 | Jacksonville State | BUF | 18 05 154 | 8 |
| 91 | Okuayinonu, Sam | DE | 05/01/1998 | 27 | 6' 01" | 269 | Maryland | TEN FA | 22 CFA | 3 |
| 1 | Pearsall, Ricky | WR | 09/09/2000 | 24 | 6' 01" | 189 | Florida | SF | 24 01 031 | 2 |
| 25 | Pinnock, Jason | S | 06/30/1999 | 26 | 6' 00" | 200 | Pittsburgh | NYJ | 21 05 175 | 5 |
| 62 | Pleasants, Austen | OT | 08/22/1997 | 28 | 6' 06" | 330 | Ohio | JAX FA | 20 CFA | 1 |
| 77 | Puni, Dominick | OG | 12/24/2000 | 24 | 6' 05" | 313 | Kansas | SF | 24 03 086 | 2 |
| 13 | Purdy, Brock | QB | 12/27/1999 | 25 | 6' 01" | 220 | Iowa State | SF | 22 07 262 | 4 |
| 30 | Robinson Jr., Brian | RB | 03/22/1999 | 26 | 6' 02" | 225 | Alabama | WAS | 22 03 098 | 4 |
| 36 | Sigle, Marques | S | 07/18/2002 | 23 | 5' 11" | 199 | Kansas State | SF | 25 05 160 | R |
| 20 | Stout, Upton | CB | 01/09/2002 | 23 | 5' 08" | 181 | Western Kentucky | SF | 25 03 100 | R |
| 88 | Tonges, Jake | TE | 07/08/1999 | 26 | 6' 04" | 240 | California | CHI FA | 22 CFA | 3 |
| 54 | Warner, Fred | LB | 11/19/1996 | 28 | 6' 03" | 230 | BYU | SF | 18 03 070 | 8 |
| 17 | Watkins, Jordan | WR | 02/15/2002 | 23 | 5' 11" | 196 | Mississippi | SF | 25 04 138 | R |
| 46 | Weeks, Jon | LS | 02/17/1986 | 39 | 5' 10" | 242 | Baylor | HOU FA | 10 CFA | 16 |
| 99 | West, C.J. | DT | 06/12/2002 | 23 | 6' 01" | 316 | Indiana | SF | 25 04 113 | R |
| 98 | Williams, Mykel | DE | 06/29/2004 | 21 | 6' 05" | 267 | Georgia | SF | 25 01 011 | R |
| 71 | Williams, Trent | OT | 08/19/1988 | 37 | 6' 05" | 320 | Oklahoma | WAS | 10 01 004 | 16 |
| 53 | Winters, Dee | LB | 10/17/2000 | 24 | 5' 11" | 227 | TCU | SF | 23 06 216 | 3 |
| Practice Squad |
| 30 | Apple, Eli | CB | 08/09/1995 | 30 | 6' 01" | 203 | Ohio State | NYG | 16 01 010 | 10 |
| 86 | Bergen, Junior | WR | 10/23/2002 | 22 | 5' 09" | 184 | Montana | SF | 25 07 252 | R |
| 69 | Bradley-King, William | DE | 12/22/1997 | 27 | 6' 03" | 265 | Baylor | WAS | 21 07 240 | 1 |
| 40 | Canteen, Derrick | S | 04/02/2001 | 24 | 5' 11" | 193 | Cincinnati | SF FA | 25 CFA | R |
| 3 | Chosen, Robbie | WR | 05/09/1993 | 32 | 6' 03" | 185 | Temple | NYJ FA | 16 CFA | 9 |
| 56 | Gipson, Trevis | DE | 06/13/1997 | 28 | 6' 03" | 263 | Tulsa | CHI | 20 05 155 | 6 |
| 41 | Graham, Jalen | LB | 01/11/2001 | 24 | 6' 02" | 220 | Purdue | SF | 23 07 255 | 3 |
| Martinez, Adrian | QB | 01/07/2000 | 25 | 6' 02" | 220 | Kansas State | DET FA | 23 CFA | 1 |
| McCormick, Sincere | RB | 09/10/2000 | 24 | 5' 08" | 204 | UTSA | LV FA | 22 CFA | 3 |
| 66 | Nugent, Drake | OC | 02/09/2001 | 24 | 6' 01" | 300 | Michigan | SF FA | 24 CFA | 1 |
| 59 | Robinson, Curtis | LB | 06/02/1998 | 27 | 6' 03" | 235 | Stanford | DEN FA | 21 CFA | 4 |
| 7 | Turner, Malik | WR | 01/30/1996 | 29 | 6' 02" | 202 | Illinois | SEA FA | 18 CFA | 5 |
| Valdes-Scantling, Marquez | WR | 10/10/1994 | 30 | 6' 04" | 206 | South Florida | GB | 18 05 174 | 8 |
| 55 | Valdez, Sebastian | DT | 08/01/2002 | 23 | 6' 04" | 301 | Washington | SF FA | 25 CFA | R |
| 9 | Willis, Brayden | TE | 11/11/1999 | 25 | 6' 03" | 240 | Oklahoma | SF | 23 07 247 | 3 |
| 63 | Zakelj, Nick | OG/OC | 06/22/1999 | 26 | 6' 06" | 316 | Fordham | SF | 22 06 187 | 4 |
| Reserves |
| 11 | Aiyuk, Brandon | WR | 03/17/1998 | 27 | 6' 00" | 200 | Arizona State | SF | 20 01 025 | 6 |
| 67 | Alarcon, Isaac | OT | 07/27/1998 | 27 | 6' 07" | 320 | Monterrey Tech | DAL FA | 20 SFA | 1 |
| 22 | Brown, Tre | CB | 09/24/1997 | 27 | 5' 10" | 185 | Oklahoma | SEA | 21 04 137 | 5 |
| 19 | Cowing, Jacob | WR | 02/04/2001 | 24 | 5' 08" | 171 | Arizona | SF | 24 04 135 | 2 |
| 90 | Givens, Kevin | DT | 03/01/1997 | 28 | 6' 01" | 285 | Penn State | SF FA | 19 CFA | 6 |
| 56 | Jackson, Tarron | DE | 06/22/1998 | 27 | 6' 02" | 254 | Coastal Carolina | PHI | 21 06 191 | 3 |
| 6 | Mustapha, Malik | S | 06/25/2002 | 23 | 5' 10" | 206 | Wake Forest | SF | 24 04 124 | 2 |
| 76 | Prince, Isaiah | OT | 07/29/1997 | 28 | 6' 06" | 305 | Ohio State | MIA | 19 06 202 | 5 |
| 5 | Robinson, Demarcus | WR | 09/21/1994 | 30 | 6' 01" | 202 | Florida | KC | 16 04 126 | 10 |
| 49 | Robinson, Jakob | CB | 10/10/2000 | 24 | 5' 11" | 181 | BYU | SF FA | 25 CFA | R |
| 4 | Rourke, Kurtis | QB | 10/25/2000 | 24 | 6' 04" | 220 | Indiana | SF | 25 07 227 | R |
| 81 | Taylor, Trent | WR | 04/30/1994 | 31 | 5' 08" | 180 | Louisiana Tech | SF | 17 05 177 | 7 |
| 32 | Taylor Jr., Patrick | RB | 04/29/1998 | 27 | 6' 01" | 217 | Memphis | GB FA | 20 CFA | 5 |
| 35 | Tomlinson, Tre | CB | 01/10/2000 | 25 | 5' 08" | 177 | TCU | LAR | 23 06 182 | 3 |